FlhD/FlhC-regulated promoters analyzed by gene array and lacZ gene fusions

FEMS Microbiol Lett. 2001 Apr 1;197(1):91-7. doi: 10.1111/j.1574-6968.2001.tb10588.x.

Abstract

The Escherichia coli transcriptional regulatory complex FlhD/FlhC, initially identified as a flagella-specific activator, is a global regulator involved in many cellular processes. Using gene arrays, lacZ gene fusions and enzyme assays, eight new targets of FlhD/FlhC were recognized. These are the transporter for galactose (MglBAC), the rod-shape determination proteins (MreBCD), malate dehydrogenase, and several enzymes involved in anaerobic respiration (glycerol 3-phosphate dehydrogenase, GlpABC; periplasmic nitrate reductase, NapFAGHBC; nitrite reductase, NrfABCDEFG; dimethyl sulfoxide reductase, DmsABC; and the modulator for hydrogenases, HydNHypF).

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Escherichia coli / genetics
  • Escherichia coli / growth & development
  • Escherichia coli / metabolism*
  • Escherichia coli Proteins
  • Flagella / genetics
  • Flagella / metabolism
  • Gene Expression Profiling
  • Gene Expression Regulation, Bacterial*
  • Lac Operon / physiology
  • Malate Dehydrogenase / genetics
  • Malate Dehydrogenase / metabolism
  • Oligonucleotide Array Sequence Analysis / methods
  • Promoter Regions, Genetic / genetics
  • Promoter Regions, Genetic / physiology*
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Trans-Activators / genetics*
  • Trans-Activators / metabolism
  • Transcription, Genetic
  • beta-Galactosidase / genetics
  • beta-Galactosidase / metabolism

Substances

  • Bacterial Proteins
  • DNA-Binding Proteins
  • Escherichia coli Proteins
  • Recombinant Fusion Proteins
  • Trans-Activators
  • flhC protein, E coli
  • flhD protein, E coli
  • Malate Dehydrogenase
  • beta-Galactosidase