Characterization of Mouse Dach2, a Homologue of Drosophila Dachshund

Mech Dev. 2001 Apr;102(1-2):169-79. doi: 10.1016/s0925-4773(01)00307-0.

Abstract

The Drosophila genes eyeless, eyes absent, sine oculis and dachshund cooperate as components of a network to control retinal determination. Vertebrate homologues of these genes have been identified and implicated in the control of cell fate. We present the cloning and characterization of mouse Dach2, a homologue of dachshund. In situ hybridization studies demonstrate Dach2 expression in embryonic nervous tissues, sensory organs and limbs. This pattern is similar to mouse Dach1, suggesting a partially redundant role for these genes during development. In addition, we determine that Dach2 expression in the forebrain of Pax6 mutants and dermamyotome of Pax3 mutants is not detectably altered. Finally, genetic mapping experiments place mouse Dach2 on the X chromosome between Xist and Esx1. The identification of human DACH2 sequences at Xq21 suggests a possible role for this gene in Allan-Herndon syndrome, Miles-Carpenter syndrome, X-linked cleft palate and/or Megalocornea.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA, Complementary / metabolism
  • DNA-Binding Proteins / biosynthesis
  • Drosophila
  • Drosophila Proteins*
  • Eye Proteins
  • Genotype
  • Homeodomain Proteins / biosynthesis
  • Humans
  • In Situ Hybridization
  • Mice
  • Mice, Inbred C57BL
  • Models, Genetic
  • Molecular Sequence Data
  • Nervous System / embryology
  • Nuclear Proteins / biosynthesis
  • Nuclear Proteins / chemistry*
  • Nuclear Proteins / genetics*
  • PAX3 Transcription Factor
  • PAX6 Transcription Factor
  • Paired Box Transcription Factors
  • Protein Structure, Tertiary
  • Proto-Oncogene Proteins / biosynthesis
  • RNA / metabolism
  • RNA, Long Noncoding
  • RNA, Untranslated / biosynthesis
  • Repressor Proteins
  • Sequence Homology, Amino Acid
  • Time Factors
  • Tissue Distribution
  • Transcription Factors / biosynthesis
  • X Chromosome

Substances

  • DACH2 protein, human
  • DNA, Complementary
  • DNA-Binding Proteins
  • Dach2 protein, mouse
  • Drosophila Proteins
  • Esx1 protein, mouse
  • Eye Proteins
  • Homeodomain Proteins
  • Nuclear Proteins
  • PAX3 Transcription Factor
  • PAX3 protein, human
  • PAX6 Transcription Factor
  • PAX6 protein, human
  • Paired Box Transcription Factors
  • Pax6 protein, mouse
  • Proto-Oncogene Proteins
  • RNA, Long Noncoding
  • RNA, Untranslated
  • Repressor Proteins
  • Transcription Factors
  • XIST non-coding RNA
  • dac protein, Drosophila
  • Pax3 protein, mouse
  • RNA