Industrial choices for protein production by large-scale cell culture

Curr Opin Biotechnol. 2001 Apr;12(2):180-7. doi: 10.1016/s0958-1669(00)00197-x.


Traditional barriers to large-scale mammalian culture have now been addressed, with the standard stirred-tank reactor emerging as industry's technology of choice. The issues of adapting cells to suspension culture, shear sensitivity and oxygen supply have been largely resolved. But for many low-volume and specialty applications, such as the production of viral vaccines and gene therapies, reactor technology remains diversified, with reactor types ranging from roller bottles to stacked plates and hollow fibers.

Publication types

  • Review

MeSH terms

  • Animals
  • Bioreactors / classification
  • Cell Culture Techniques / methods*
  • Cells, Cultured
  • Drug Industry
  • Recombinant Proteins / biosynthesis*
  • Recombinant Proteins / genetics
  • United States
  • United States Food and Drug Administration


  • Recombinant Proteins