Histone acetyltransferase complexes stabilize swi/snf binding to promoter nucleosomes

Cell. 2001 Mar 23;104(6):817-27. doi: 10.1016/s0092-8674(01)00279-3.


To investigate the function of SWI/SNF in site-specific chromatin remodeling at promoters, we have used a purified system to analyze its distribution, function, and retention following recruitment by a sequence-specific transcription activator. Activator recruitment of SWI/SNF bound the complex to promoter proximal nucleosomes and led to localized nucleosome disruption. However, retention of SWI/SNF on the promoter required either the continued binding of the transcription activator or acetylated histones. Histone acetylation by either the SAGA or NuA4 HAT complexes increased the retention of SWI/SNF on the promoter. These data illustrate a functional link between HAT complexes and the SWI/SNF chromatin remodeling complex and provide a mechanistic basis for the ordered recruitment of these complexes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetyltransferases / chemistry
  • Acetyltransferases / metabolism*
  • Binding Sites
  • Chromatin / metabolism
  • Chromatin / ultrastructure
  • Chromosomal Proteins, Non-Histone
  • Fungal Proteins / isolation & purification
  • Fungal Proteins / metabolism
  • Histone Acetyltransferases
  • Nucleosomes / genetics*
  • Nucleosomes / metabolism*
  • Promoter Regions, Genetic*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins*
  • Transcription Factors / isolation & purification
  • Transcription Factors / metabolism*


  • Chromatin
  • Chromosomal Proteins, Non-Histone
  • Fungal Proteins
  • Nucleosomes
  • SAGA protein, Emericella nidulans
  • SWI1 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Acetyltransferases
  • Histone Acetyltransferases