A common class of nematode glutathione S-transferase (GST) revealed by the theoretical proteome of the model organism Caenorhabditis elegans

Comp Biochem Physiol B Biochem Mol Biol. 2001 Apr;128(4):701-8. doi: 10.1016/s1096-4959(00)00360-2.

Abstract

The genome verified C. elegans free-living nematode model is a new tool for investigating gene expression in human and animal nematode parasites. There is limited information on designating glutathione S-transferase (GST) to specific classes in lower invertebrates such as nematodes. Following cloning, amino acid sequence alignment, recombinant expression and Western blotting we provide evidence of a new GST class in nematodes or lower invertebrates.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Caenorhabditis elegans / enzymology*
  • Caenorhabditis elegans Proteins / chemistry
  • Cloning, Molecular
  • Glutathione Transferase / chemistry
  • Glutathione Transferase / classification*
  • Kinetics
  • Mice
  • Models, Molecular
  • Molecular Sequence Data
  • Nematoda / enzymology*
  • Parasites / enzymology
  • Phylogeny
  • Proteome* / chemistry
  • Sequence Alignment
  • Substrate Specificity

Substances

  • Caenorhabditis elegans Proteins
  • Proteome
  • Glutathione Transferase

Associated data

  • GENBANK/AF128959