c-Myb and Bcl-x overexpression predicts poor prognosis in colorectal cancer: clinical and experimental findings

Am J Pathol. 2001 Apr;158(4):1289-99. doi: 10.1016/S0002-9440(10)64080-1.


The aim of this study was twofold: to assess the relationship between c-Myb and Bcl-x expression and to evaluate the prognostic significance of their expression in colorectal carcinoma (CRC) patients. Analysis of tumors from 91 CRC patients for expression of c-Myb and Bcl-x revealed a significant relationship between these two proteins. Kaplan-Meier's analysis showed an increased risk of relapse and death in patients whose tumor specimens displayed high c-Myb levels and Bcl-x positivity. Similar results were also observed excluding Dukes' D patients. Molecular analysis using three c-Myb-overexpressing LoVo clones indicated that c-Myb overexpression was accompanied by up-regulation of Bcl-x(L) protein and mRNA. Tumors originating from these clones injected in nude mice were significantly larger than those formed in mice injected with parental or vector-transfected LoVo cells. Moreover, tumors derived from parental and control vector-transfected but not from c-Myb-overexpressing LoVo cells showed high frequency of apoptotic cells. These results provide direct evidence of an association between c-Myb and Bcl-x expression and suggest that expression of both molecules might be a useful prognostic marker in CRC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carcinoma / pathology
  • Carcinoma / physiopathology*
  • Cell Division / physiology
  • Colonic Neoplasms / pathology
  • Colonic Neoplasms / physiopathology*
  • Humans
  • Prognosis
  • Proto-Oncogene Proteins c-bcl-2 / genetics
  • Proto-Oncogene Proteins c-bcl-2 / metabolism*
  • Proto-Oncogene Proteins c-myb / genetics
  • Proto-Oncogene Proteins c-myb / metabolism*
  • RNA, Messenger / metabolism
  • Rectal Neoplasms / pathology
  • Rectal Neoplasms / physiopathology*
  • Survival Analysis
  • Transfection
  • bcl-X Protein


  • BCL2L1 protein, human
  • Proto-Oncogene Proteins c-bcl-2
  • Proto-Oncogene Proteins c-myb
  • RNA, Messenger
  • bcl-X Protein