Role of the anterior visceral endoderm in restricting posterior signals in the mouse embryo

Int J Dev Biol. 2001;45(1):311-20.


Recent genetic and embryological experiments have demonstrated that head formation in the mouse embryo is dependent on signals provided by two organising centers during gastrulation, the anterior visceral endoderm (AVE) and the anterior primitive streak (also called the Early Gastrula Organiser, EGO). However the molecular nature of the signals triggering anterior neural formation from the epiblast is not clearly understood. The analysis of mouse mutants has allowed the identification of some of the molecular players involved in the process of head formation. In this review, we describe different mutant embryos in which impairment of visceral endoderm function leads to similar defects in antero-posterior axis specification. These phenotypes are consistent with a role of the AVE in protecting anterior embryonic regions from signals that promote posterior development. We propose that a genetic cascade in the AVE, involving HNF3beta, Lim1, Otx2, Smad2 and ActRIB, leads to the production of secreted TGFbeta antagonists that protect the anterior epiblast region from Nodal signalling.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Activin Receptors, Type I
  • Animals
  • Arabidopsis Proteins*
  • Body Patterning
  • Fetal Proteins*
  • Gastrula / cytology
  • Gene Expression Regulation, Developmental
  • Homeodomain Proteins*
  • Left-Right Determination Factors
  • Mice
  • Models, Biological
  • Mutation
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / physiology
  • Nodal Protein
  • Organizers, Embryonic*
  • Otx Transcription Factors
  • Plant Proteins / genetics
  • Plant Proteins / physiology
  • Receptors, Growth Factor / genetics
  • Receptors, Growth Factor / physiology
  • Signal Transduction
  • T-Box Domain Proteins / genetics
  • T-Box Domain Proteins / physiology
  • Trans-Activators / genetics
  • Trans-Activators / physiology
  • Transforming Growth Factor beta / antagonists & inhibitors
  • Transforming Growth Factor beta / genetics
  • Transforming Growth Factor beta / physiology


  • Arabidopsis Proteins
  • CER protein, Arabidopsis
  • Fetal Proteins
  • Homeodomain Proteins
  • Left-Right Determination Factors
  • Nerve Tissue Proteins
  • Nodal Protein
  • Nodal protein, mouse
  • Otx Transcription Factors
  • Otx2 protein, mouse
  • Plant Proteins
  • Receptors, Growth Factor
  • T-Box Domain Proteins
  • Trans-Activators
  • Transforming Growth Factor beta
  • Activin Receptors, Type I
  • Brachyury protein