Overexpression of Lymphangiogenic Growth Factor VEGF-C in Human Pancreatic Cancer

Pancreas. 2001 Apr;22(3):285-92. doi: 10.1097/00006676-200104000-00010.

Abstract

Vascular endothelial growth factor C (VEGF-C) is a lymphangiogenic polypeptide that has been implicated in cancer growth. In this study, we characterized VEGF-C expression in cultured human pancreatic cancer cell lines and determined whether the presence of VEGF-C in human pancreatic cancers is associated with clinicopathologic characteristics. VEGF-C mRNA transcripts were present in all five tested cell lines (Capan-1, MIA-PaCa-2, PANC-1, COLO-357, and T3M4). Immunoblotting with a highly specific anti-VEGF-C antibody revealed the presence of VEGF-C protein in all the cell lines. Northern blot analysis of total RNA revealed an approximately 2.2-fold increase in VEGF-C mRNA transcript in the cancer samples compared with the normal pancreas. Immunohistochemical analysis confirmed the expression of VEGF-C and its receptor flt-4 in the cancer cells within the tumor mass. Immunohistochemical analysis of 51 pancreatic cancer tissues revealed the presence of strong VEGF-C immunoreactivity in the cancer cells in 80.4% of the cancer tissues. The presence of VEGF-C in these cells was associated with increased lymphatic vessels invasion and lymph node metastasis, but not with decreased patient survival. These findings indicate that VEGF-C and its receptor are commonly overexpressed in human pancreatic cancers and that this factor may contribute to the lymphangiogenic process and metastasis in this disorder.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Northern
  • Endothelial Growth Factors / genetics*
  • Humans
  • Immunoblotting
  • Immunohistochemistry
  • Lymphatic Metastasis
  • Neoplasm Invasiveness
  • Pancreas / chemistry
  • Pancreatic Neoplasms / chemistry*
  • Pancreatic Neoplasms / pathology
  • RNA, Messenger / analysis*
  • Receptor Protein-Tyrosine Kinases / analysis
  • Receptors, Growth Factor / analysis
  • Survival Rate
  • Tumor Cells, Cultured
  • Vascular Endothelial Growth Factor C
  • Vascular Endothelial Growth Factor Receptor-3

Substances

  • Endothelial Growth Factors
  • RNA, Messenger
  • Receptors, Growth Factor
  • Vascular Endothelial Growth Factor C
  • Receptor Protein-Tyrosine Kinases
  • Vascular Endothelial Growth Factor Receptor-3