Molecular epidemiology of methicillin-resistant Staphylococcus aureus

Emerg Infect Dis. Mar-Apr 2001;7(2):323-6. doi: 10.3201/eid0702.010236.


Subtyping methicillin- resistant Staphylococcus aureus (MRSA) isolates and tracking nosocomial infections have evolved from phenotypic to genotypic approaches; most laboratories now depend on pulsed-field gel electrophoresis (PFGE). We discuss the limitations of current image-based genotyping methods, including PFGE, and the advantages (including ease of entering data into a database) of using DNA sequence analysis to control MRSA infections in health-care facilities.

Publication types

  • Review

MeSH terms

  • Bacterial Typing Techniques / methods
  • DNA, Bacterial / analysis
  • Genotype
  • Humans
  • Methicillin Resistance*
  • Sequence Analysis, DNA / methods
  • Staphylococcal Infections / epidemiology
  • Staphylococcal Infections / microbiology
  • Staphylococcus aureus / classification
  • Staphylococcus aureus / drug effects
  • Staphylococcus aureus / genetics*
  • Staphylococcus aureus / isolation & purification


  • DNA, Bacterial