Abstract
p53-binding consensus-like sequence (T3SF) is located in the murine promoter region of tissue inhibitor of metalloproteinase 3 gene. To identify the genes that encode proteins that bind to T3SF DNA sequence, we screened a cDNA library using the Southwestern technique. The SMRT gene was cloned as one of the candidates. Addition of antibody against SMRT reduced the intensity of a band that is supposed to contain SMRT in electrophoresis mobility shift assay, although antibody against p53 had no effect. Ultraviolet (UV)-irradiation reduced the intensity of the SMRT complex whereas p53 complex was stabilized by UV-irradiation. These results suggest that SMRT may bind to T3SF sequence in p53-independent manner and dissociate from the sequence by UV irradiation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Binding Sites
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Blotting, Southern
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Blotting, Western
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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DNA-Binding Proteins / radiation effects
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Electrophoresis, Agar Gel
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Gene Library
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Humans
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Nuclear Receptor Co-Repressor 2
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Repressor Proteins / genetics
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Repressor Proteins / metabolism*
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Repressor Proteins / radiation effects
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Tissue Inhibitor of Metalloproteinase-3 / genetics
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Tissue Inhibitor of Metalloproteinase-3 / metabolism
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Tumor Cells, Cultured / metabolism*
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Tumor Cells, Cultured / radiation effects
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Tumor Suppressor Protein p53 / genetics
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Tumor Suppressor Protein p53 / metabolism*
Substances
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DNA-Binding Proteins
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NCOR2 protein, human
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Nuclear Receptor Co-Repressor 2
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Repressor Proteins
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Tissue Inhibitor of Metalloproteinase-3
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Tumor Suppressor Protein p53