SMRT as a T3SF-binding protein

Int J Oncol. 2001 May;18(5):985-9. doi: 10.3892/ijo.18.5.985.

Abstract

p53-binding consensus-like sequence (T3SF) is located in the murine promoter region of tissue inhibitor of metalloproteinase 3 gene. To identify the genes that encode proteins that bind to T3SF DNA sequence, we screened a cDNA library using the Southwestern technique. The SMRT gene was cloned as one of the candidates. Addition of antibody against SMRT reduced the intensity of a band that is supposed to contain SMRT in electrophoresis mobility shift assay, although antibody against p53 had no effect. Ultraviolet (UV)-irradiation reduced the intensity of the SMRT complex whereas p53 complex was stabilized by UV-irradiation. These results suggest that SMRT may bind to T3SF sequence in p53-independent manner and dissociate from the sequence by UV irradiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Blotting, Southern
  • Blotting, Western
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • DNA-Binding Proteins / radiation effects
  • Electrophoresis, Agar Gel
  • Gene Library
  • Humans
  • Nuclear Receptor Co-Repressor 2
  • Repressor Proteins / genetics
  • Repressor Proteins / metabolism*
  • Repressor Proteins / radiation effects
  • Tissue Inhibitor of Metalloproteinase-3 / genetics
  • Tissue Inhibitor of Metalloproteinase-3 / metabolism
  • Tumor Cells, Cultured / metabolism*
  • Tumor Cells, Cultured / radiation effects
  • Tumor Suppressor Protein p53 / genetics
  • Tumor Suppressor Protein p53 / metabolism*

Substances

  • DNA-Binding Proteins
  • NCOR2 protein, human
  • Nuclear Receptor Co-Repressor 2
  • Repressor Proteins
  • Tissue Inhibitor of Metalloproteinase-3
  • Tumor Suppressor Protein p53