THUMP--a predicted RNA-binding domain shared by 4-thiouridine, pseudouridine synthases and RNA methylases

Trends Biochem Sci. 2001 Apr;26(4):215-7. doi: 10.1016/s0968-0004(01)01826-6.


Sequence profile searches were used to identify an ancient domain in ThiI-like thiouridine synthases, conserved RNA methylases, archaeal pseudouridine synthases and several uncharacterized proteins. We predict that this domain is an RNA-binding domain that adopts an alpha/beta fold similar to that found in the C-terminal domain of translation initiation factor 3 and ribosomal protein S8.

MeSH terms

  • Amino Acid Sequence
  • Binding Sites
  • Hydro-Lyases / metabolism*
  • Molecular Sequence Data
  • RNA / metabolism*
  • RNA-Binding Proteins / chemistry
  • RNA-Binding Proteins / metabolism*
  • Sequence Homology, Amino Acid
  • Thiouridine / metabolism*
  • tRNA Methyltransferases / metabolism*


  • RNA-Binding Proteins
  • Thiouridine
  • RNA
  • tRNA Methyltransferases
  • Hydro-Lyases
  • pseudouridylate synthetase