Curcuminoids from Curcuma longa L. (Zingiberaceae) that protect PC12 rat pheochromocytoma and normal human umbilical vein endothelial cells from betaA(1-42) insult

Neurosci Lett. 2001 Apr 27;303(1):57-61. doi: 10.1016/s0304-3940(01)01677-9.

Abstract

beta-Amyloid (betaA) induced oxidative stress is a well-established pathway of neuronal cell death in Alzheimer's disease. From turmeric, Curcuma longa L. (Zingiberaceae), three curcuminoids, curcumin, demethoxycurcumin, and bisdemethoxycurcumin, were found to protect PC12 rat pheochromocytoma and normal human umbilical vein endothelial (HUVEC) cells from betaA(1-42) insult, as measured by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide reduction assay. ED(50) values of curcumin, demethoxycurcumin, and bisdemethoxycurcumin toward PC12 and HUVEC cells were 7.1+/-0.3, 4.7+/-0.1, 3.5+/-0.2 microg/ml and 6.8+/-0.4, 4.2+/-0.3, and 3.0+/-0.3 microg/ml, respectively. These compounds were better antioxidants than alpha-tocopherol as determined by DPPH radical trapping experiment. alpha-Tocopherol did not protect the cells from betaA(1-42) insult even at>50 microg/ml concentration. The results suggest that these compounds may be protecting the cells from betaA(1-42) insult through antioxidant pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alzheimer Disease / physiopathology
  • Amyloid beta-Peptides / pharmacology*
  • Animals
  • Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
  • Antioxidants / pharmacology
  • Cell Survival / drug effects
  • Cell Survival / physiology
  • Coumaric Acids / pharmacology*
  • Curcumin / analogs & derivatives
  • Curcumin / pharmacology*
  • Diarylheptanoids
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / drug effects*
  • Endothelium, Vascular / physiology
  • Humans
  • PC12 Cells
  • Peptide Fragments / pharmacology*
  • Rats
  • Umbilical Veins / cytology
  • Umbilical Veins / drug effects
  • Umbilical Veins / physiology
  • Zingiberales

Substances

  • Amyloid beta-Peptides
  • Anti-Inflammatory Agents, Non-Steroidal
  • Antioxidants
  • Coumaric Acids
  • Diarylheptanoids
  • Peptide Fragments
  • amyloid beta-protein (1-42)
  • bis(4-hydroxycinnamoyl)methane
  • Curcumin
  • demethoxycurcumin