Structural insights into the molecular mechanism of calcium-dependent vesicle-membrane fusion

Curr Opin Struct Biol. 2001 Apr;11(2):163-73. doi: 10.1016/s0959-440x(00)00186-x.

Abstract

The fusion of vesicles with target membranes is controlled by a complex network of protein-protein and protein-lipid interactions. Recently determined structures of the SNARE complex, synaptotagmin III, nSec1, domains of the NSF chaperone and its adaptor (SNAP), and Rab3 and some of its effectors provide the framework for developing molecular models of vesicle fusion and for designing experiments to test these models. Ultimately, knowledge of the structures of higher-order complexes and their dynamic behavior will be required to obtain a full understanding of the vesicle fusion protein machinery.

Publication types

  • Review

MeSH terms

  • Animals
  • Calcium / metabolism*
  • Calcium-Binding Proteins*
  • Carrier Proteins / chemistry
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism
  • Membrane Fusion / physiology*
  • Membrane Glycoproteins / chemistry
  • Membrane Glycoproteins / metabolism*
  • Membrane Proteins / chemistry
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism
  • Membrane Proteins / physiology*
  • N-Ethylmaleimide-Sensitive Proteins
  • Nerve Tissue Proteins / chemistry
  • Nerve Tissue Proteins / metabolism*
  • SNARE Proteins
  • Saccharomyces cerevisiae Proteins*
  • Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
  • Synaptotagmins
  • Transport Vesicles / chemistry
  • Transport Vesicles / metabolism*
  • Vesicular Transport Proteins*
  • rab3A GTP-Binding Protein / genetics
  • rab3A GTP-Binding Protein / metabolism

Substances

  • Calcium-Binding Proteins
  • Carrier Proteins
  • Membrane Glycoproteins
  • Membrane Proteins
  • Nerve Tissue Proteins
  • SEC17 protein, S cerevisiae
  • SNARE Proteins
  • Saccharomyces cerevisiae Proteins
  • Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
  • Vesicular Transport Proteins
  • Synaptotagmins
  • N-Ethylmaleimide-Sensitive Proteins
  • rab3A GTP-Binding Protein
  • Calcium