Plasma cholesteryl ester transfer and hepatic lipase activity are related to high-density lipoprotein cholesterol in association with insulin resistance in type 2 diabetic and non-diabetic subjects

Scand J Clin Lab Invest. 2001 Feb;61(1):1-9. doi: 10.1080/00365510151067866.

Abstract

We evaluated the hypothesis that plasma cholesteryl ester transfer (CET) and lipase activities are influenced by insulin sensitivity and contribute to the low high-density lipoprotein (HDL) cholesterol observed in type 2 diabetic patients and insulin-resistant non-diabetic subjects. Sixteen type 2 diabetic and 16 non-diabetic subjects participated. Diabetic and non-diabetic subjects were divided in equal groups of eight subjects with low or high insulin sensitivity, which was documented as the glucose infusion rate (M-value) during the last hour of a 3-h euglycaemic hyperinsulinaemic clamp (150 mU kg(-1) h(-1), blood glucose target 4.6 mmol L(-1)). Post-heparin plasma lipoprotein lipase (LPL) and hepatic lipase (HL) activities were measured in samples obtained 1-2 weeks before the clamp. Plasma CET was measured by a radioisotope method. Compared to non-diabetic men with high insulin sensitivity (n = 8) HDL cholesterol was lower in type 2 diabetic men (n=8, p<0.01) and non-diabetic men (n=8, p <0.05) with low insulin sensitivity, and the HDL cholesterylester content was lower in type 2 diabetic men with high insulin sensitivity (n=8, p<0.05). In non-diabetic subjects with high insulin sensitivity, plasma CET was lower than in the other groups (p<0.05 for all). Multiple regression analysis showed that plasma CET (p=0.001) and HL activity (p=0.02) were independently and negatively associated with the M-value. No association between the M-value and LPL activity was observed. Independent negative relationships of HDL cholesterol with plasma CET (p = 0.04) and HL activity (p=0.03) were observed. This study supports the hypothesis that a low HDL cholesterol associated with insulin resistance in type 2 diabetic and non-diabetic subjects is related to a high plasma CET and a high HL activity.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Apolipoproteins B / blood
  • Biological Transport
  • Body Mass Index
  • Cholesterol Esters / blood*
  • Cholesterol, HDL / blood*
  • Cholesterol, LDL / blood
  • Cholesterol, VLDL / blood
  • Diabetes Mellitus, Type 2 / blood*
  • Esterification
  • Fatty Acids, Nonesterified / blood
  • Glycated Hemoglobin A / analysis
  • Humans
  • Insulin Resistance*
  • Lipase / blood*
  • Liver / enzymology*
  • Middle Aged
  • Triglycerides / blood

Substances

  • Apolipoproteins B
  • Cholesterol Esters
  • Cholesterol, HDL
  • Cholesterol, LDL
  • Cholesterol, VLDL
  • Fatty Acids, Nonesterified
  • Glycated Hemoglobin A
  • Triglycerides
  • Lipase