TRPC1 and TRPC5 form a novel cation channel in mammalian brain

Neuron. 2001 Mar;29(3):645-55. doi: 10.1016/s0896-6273(01)00240-9.


TRP proteins are cation channels responding to receptor-dependent activation of phospholipase C. Mammalian (TRPC) channels can form hetero-oligomeric channels in vitro, but native TRPC channel complexes have not been identified to date. We demonstrate here that TRPC1 and TRPC5 are subunits of a heteromeric neuronal channel. Both TRPC proteins have overlapping distributions in the hippocampus. Coexpression of TRPC1 and TRPC5 in HEK293 cells resulted in a novel nonselective cation channel with a voltage dependence similar to NMDA receptor channels, but unlike that of any reported TRPC channel. TRPC1/TRPC5 heteromers were activated by G(q)-coupled receptors but not by depletion of intracellular Ca(2+) stores. In contrast to the more common view of the TRP family as comprising store-operated channels, we propose that many TRPC heteromers form diverse receptor-regulated nonselective cation channels in the mammalian brain.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Axons / chemistry
  • Brain Chemistry*
  • Calcium / analysis
  • Calcium Channels / analysis
  • Calcium Channels / chemistry*
  • Calcium Channels / genetics
  • Cation Transport Proteins*
  • Cations
  • Cell Line
  • Dendrites / chemistry
  • Electric Conductivity
  • Embryo, Mammalian
  • Gene Expression
  • Hippocampus / chemistry
  • Humans
  • Ion Channels / chemistry*
  • Kidney
  • Macromolecular Substances
  • Neurons / chemistry
  • Neurons / ultrastructure
  • Rats
  • Receptors, N-Methyl-D-Aspartate / physiology
  • TRPC Cation Channels
  • Transfection


  • Calcium Channels
  • Cation Transport Proteins
  • Cations
  • Ion Channels
  • Macromolecular Substances
  • Receptors, N-Methyl-D-Aspartate
  • TRPC Cation Channels
  • TRPC5 protein, human
  • Trpc5 protein, rat
  • transient receptor potential cation channel, subfamily C, member 1
  • Calcium