Identification of Ras-regulated genes by representational difference analysis

J M Shields; C J Der; S Powers

doi:10.1016/s0076-6879(01)32205-x

Identification of Ras-regulated genes by representational difference analysis

Methods Enzymol. 2001:332:221-32. doi: 10.1016/s0076-6879(01)32205-x.

Authors

J M Shields¹, C J Der, S Powers

Affiliation

¹ Department of Pharmacology, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599-7295, USA.

PMID: 11305099
DOI: 10.1016/s0076-6879(01)32205-x

Abstract

In conclusion, RDA provides a fast, technically simple, and inexpensive way to characterize genes aberrantly expressed due to Ras transformation. The identification and characterization of these genes may provide insight not only into the mechanism by which Ras causes transformation, but also may identify novel targets for rational drug design and development of anticancer drugs.

Publication types

Comparative Study

MeSH terms

Animals
Base Sequence
Cell Line
Cloning, Molecular
DNA Primers / genetics
DNA, Complementary / genetics
DNA, Complementary / isolation & purification
Down-Regulation
Gene Expression Regulation
Genes, ras*
Genetic Techniques*
Nucleic Acid Hybridization
Polymerase Chain Reaction
RNA, Messenger / genetics
RNA, Messenger / isolation & purification
Rats

Substances

DNA Primers
DNA, Complementary
RNA, Messenger