Protein transduction: delivery of Tat-GTPase fusion proteins into mammalian cells

Methods Enzymol. 2001;332:36-49. doi: 10.1016/s0076-6879(01)32190-0.
No abstract available

MeSH terms

  • Animals
  • Chromatography, High Pressure Liquid
  • Chromatography, Ion Exchange
  • Fluorescein-5-isothiocyanate
  • GTP Phosphohydrolases / genetics*
  • GTP Phosphohydrolases / isolation & purification
  • GTP Phosphohydrolases / metabolism*
  • Gene Products, tat / genetics*
  • Gene Products, tat / isolation & purification
  • Gene Products, tat / metabolism*
  • Genetic Vectors
  • Humans
  • Osteoclasts / metabolism
  • Osteoclasts / ultrastructure
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / isolation & purification
  • Recombinant Fusion Proteins / metabolism
  • Transduction, Genetic / methods*
  • rho GTP-Binding Proteins / genetics
  • rho GTP-Binding Proteins / isolation & purification
  • rho GTP-Binding Proteins / metabolism

Substances

  • Gene Products, tat
  • Recombinant Fusion Proteins
  • GTP Phosphohydrolases
  • rho GTP-Binding Proteins
  • Fluorescein-5-isothiocyanate