Hydrogen peroxide-induced changes in intracellular pH of guard cells precede stomatal closure

Cell Res. 2001 Mar;11(1):37-43. doi: 10.1038/sj.cr.7290064.


Epidermal bioassay demonstrated that benzylamine, a membrane-permeable weak base, can mimick hydrogen peroxide (H2O2) to induce stomatal closure, and butyric acid, a membrane-permeable weak acid, can partly abolish the H2O2-induced stomatal closure. Confocal pH mapping with the probe 5-(and-6)-carboxy seminaphthorhodafluor-1-acetoxymethylester (SNARF-1-AM) revealed that H2O2 leads to rapid changes in cytoplasmic and vacuolar pH in guard cells of Vicia faba L, i. e. alkalinization of cytoplasmic areas occur red in parallel with a decrease of the vacuolar pH, and that butyric acid pretreatment can abolish alkalinization of cytoplasmic areas and acidification of vacuolar areas of guard cells challenged with H2O2. These results imply that the alkalinization of cytoplasm via efflux of cytosol protons into the vacuole in guard cells challenged with H2O2 is important at an early stage in the signal cascade leading to stomatal closure.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzopyrans
  • Benzylamines / pharmacology
  • Butyric Acid / pharmacology
  • Cytoplasm / drug effects
  • Cytoplasm / metabolism*
  • Fabaceae / cytology
  • Fabaceae / drug effects*
  • Fabaceae / metabolism*
  • Fluorescent Dyes / pharmacology
  • Free Radicals / metabolism
  • Histamine Antagonists / pharmacology
  • Hydrogen Peroxide / pharmacology*
  • Hydrogen-Ion Concentration / drug effects
  • Intracellular Fluid / drug effects
  • Intracellular Fluid / metabolism*
  • Naphthols / pharmacology
  • Oxidative Stress / drug effects
  • Oxidative Stress / physiology*
  • Plant Physiological Phenomena / drug effects
  • Plant Structures / drug effects
  • Plant Structures / metabolism*
  • Plant Structures / ultrastructure
  • Plants, Medicinal*
  • Rhodamines / pharmacology
  • Signal Transduction / drug effects
  • Signal Transduction / physiology*


  • Benzopyrans
  • Benzylamines
  • Fluorescent Dyes
  • Free Radicals
  • Histamine Antagonists
  • Naphthols
  • Rhodamines
  • seminaphthorhodaminefluoride
  • Butyric Acid
  • Hydrogen Peroxide