Growth-inhibitory effect of adenovirus-mediated p53 gene transfer on medulloblastoma cell line, Daoy, harboring mutant p53

Childs Nerv Syst. 2001 Feb;17(3):134-8. doi: 10.1007/s003810000341.


To improve the survival rate, gene therapy, such as the replacement of inactivated tumor suppressor genes, has become a new investigational adjuvant treatment modality for human malignancies. We investigated the effect of adenovirus(Ad)-mediated transfer of wildtype p53 tumor suppressor gene on the medulloblastoma cell line, Daoy, which harbors mutant-type p53 gene. At 50 multiplicity of infection (moi), immunohistochemical staining with p53 monoclonal antibody showed positive staining in all cells 2 days after Ad-CMV-p53 infection. The high expression of wild-type p53 protein was detected in Ad-CMV-p53-infected cells, and expression of wild-type p53 protein peaked on day 2 after the infection. The growth of Ad-CMV-p53-infected cells was greatly suppressed in vitro, and the Ad-CMV-p53 treatment significantly reduced the tumor mass in vivo. The mean weight of Ad-CMV- infected tumors was only 16% of those which were mock infected, and 25% of those which were Ad-CMV-beta-gal infected. On microscopic examination, Ad-CMV-p53-infected tumors showed numerous apoptotic bodies. This Ad-CMV-p53 gene transfer showed high transduction efficacy and expression, resulting in significant growth inhibition of Daoy harboring mutant type p53.

MeSH terms

  • Adenoviruses, Human / genetics*
  • Blotting, Western
  • Cell Division / genetics
  • Cell Line, Transformed
  • Gene Expression Regulation, Neoplastic
  • Gene Transfer Techniques*
  • Genes, Tumor Suppressor / genetics*
  • Genes, p53 / genetics*
  • Genetic Therapy / methods*
  • Humans
  • Medulloblastoma / genetics
  • Medulloblastoma / therapy*
  • Mutation*