Towards an understanding of complex protein networks

Trends Cell Biol. 2001 Mar;11(3):102-6. doi: 10.1016/s0962-8924(00)01902-4.


Large-scale two-hybrid screens have generated a wealth of information describing potential protein--protein interactions. When compiled with data from systematic localizations of proteins, mutant screens and other functional tests, a network of interactions among proteins and between proteins and other components of eukaryotic cells can be deduced. These networks can be viewed as maps of the cell, depicting potential signaling pathways and interactive complexes. Most importantly, they provide potential clues to the function of previously uncharacterized proteins. Focusing on recent experiments, we explore these protein-interaction studies and the maps derived from such efforts.

Publication types

  • Review

MeSH terms

  • Cell Cycle / physiology
  • Cells / metabolism
  • Computational Biology
  • Models, Biological
  • Peptide Mapping
  • Proteins / classification
  • Proteins / genetics
  • Proteins / metabolism*
  • Proteome / genetics*
  • Proteome / metabolism
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism
  • Two-Hybrid System Techniques*


  • Proteins
  • Proteome