Comparative Analysis of Human, Bovine, and Murine Oct-4 Upstream Promoter Sequences

Mamm Genome. 2001 Apr;12(4):309-17. doi: 10.1007/s003350010279.

Abstract

The Oct-4 gene encodes a transcription factor that is specifically expressed in embryonic stem cells and germ cells of the mouse embryo. Cells that differentiate into somatic tissues lose Oct-4 expression. Regulation of Oct-4 gene transcription involves a TATA-less minimal promoter and two upstream elements: the proximal (PE) and distal enhancers (DE). We report here the nucleotide sequence of the 5' upstream regulatory regions of the human and murine Oct-4 genes. A comparative alignment analysis between these regions and those of the bovine Oct-4 ortholog reveals four conserved regions of homology (CR 1 to 4) between these species (66-94% conservation). The 1A sequence within the mouse PE is located approximately half-way between CR 2 and CR 3. A putative Sp1/Sp3 binding site and the overlapping hormone responsive element (HRE) in CR1 are identical in all three species. A high number of CCC(A/T)CCC motifs exhibit various levels of homology in these upstream regions. We discuss the importance of these and other sequences and present candidate factors that may bind and regulate Oct-4 gene expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cattle
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Gene Expression Profiling
  • Humans
  • Mice
  • Molecular Sequence Data
  • Octamer Transcription Factor-3
  • Promoter Regions, Genetic*
  • Sequence Homology
  • Stem Cells
  • TATA Box
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism
  • Transfection

Substances

  • DNA-Binding Proteins
  • Octamer Transcription Factor-3
  • POU5F1 protein, human
  • Pou5f1 protein, mouse
  • Transcription Factors

Associated data

  • GENBANK/AJ297527
  • GENBANK/AJ297528