The effect of the source pressure on the abundance of ions of noncovalent protein assemblies in an electrospray ionization orthogonal time-of-flight instrument

Rapid Commun Mass Spectrom. 2001;15(8):596-601. doi: 10.1002/rcm.275.

Abstract

The effect of elevating the pressure in the interface region of an electrospray ionization orthogonal time-of-flight mass spectrometer on the ion intensity of different noncovalent protein assemblies has been investigated. Elevating the pressure in the interface region generally led to an enhanced detection of high m/z ions. The optimum pressure was found to be dependent on the m/z value of the ions. This pressure effect should be carefully addressed when relating ion abundance in the mass spectra to solution phase abundance of noncovalent protein assemblies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 4-Hydroxybenzoate-3-Monooxygenase / chemistry
  • Penicillium / enzymology
  • Pressure
  • Proteins / chemistry*
  • Pseudomonas fluorescens / enzymology
  • Spectrometry, Mass, Electrospray Ionization

Substances

  • Proteins
  • 4-Hydroxybenzoate-3-Monooxygenase