Altered expression of genes in experimentally induced myopic chick eyes

Jpn J Ophthalmol. 2001 Mar-Apr;45(2):137-43. doi: 10.1016/s0021-5155(00)00360-9.


Purpose: To identify a casual pathway between the alteration in visual experience, due to form deprivation and hyperopic defocus, and the increase in eye growth, we searched for candidate genes having regulatory effects on eye growth under myopic conditions.

Methods: The expression of the brain-derived neurotrophic factor, neurotrophin-3, sonic hedgehog, nerve growth factor, Six-3 and the Lh-2 group of genes in the transcriptional level after experimentally induced myopia (form-deprivation by goggles and by hyperopic defocus using negative spectacle lenses) were evaluated by semiquantitative reverse transcriptional polymerase chain reaction and Northern blot analysis.

Results: Results showed that only the sonic hedgehog gene was differentially expressed in the experimentally induced myopic retinal samples compared with controls.

Conclusions: The sonic hedgehog gene may have regulatory functions in the signaling of the cascade of events that leads to axial elongation and vitreous enlargement of the eye under myopic conditions.

MeSH terms

  • Animals
  • Blotting, Northern
  • Brain-Derived Neurotrophic Factor / genetics
  • Chickens
  • DNA Primers / chemistry
  • Eye Proteins / biosynthesis
  • Eye Proteins / genetics*
  • Gene Expression
  • Hedgehog Proteins
  • Homeodomain Proteins / biosynthesis
  • Homeodomain Proteins / genetics
  • Male
  • Myopia / etiology
  • Myopia / metabolism*
  • Nerve Growth Factor / genetics
  • Nerve Growth Factors / biosynthesis
  • Nerve Growth Factors / genetics*
  • Nerve Tissue Proteins / biosynthesis
  • Nerve Tissue Proteins / genetics
  • Neurotrophin 3 / genetics
  • Protein Biosynthesis
  • Proteins / genetics*
  • RNA, Messenger / biosynthesis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sensory Deprivation*
  • Trans-Activators*
  • Transcription Factors / biosynthesis
  • Transcription Factors / genetics


  • Brain-Derived Neurotrophic Factor
  • DNA Primers
  • Eye Proteins
  • Hedgehog Proteins
  • Homeodomain Proteins
  • Nerve Growth Factors
  • Nerve Tissue Proteins
  • Neurotrophin 3
  • Proteins
  • RNA, Messenger
  • Sine oculis homeobox homolog 3 protein
  • Trans-Activators
  • Transcription Factors
  • Nerve Growth Factor