Presynaptic mechanisms underlying cannabinoid inhibition of excitatory synaptic transmission in rat striatal neurons

J Physiol. 2001 May 1;532(Pt 3):731-48. doi: 10.1111/j.1469-7793.2001.0731e.x.

Abstract

The striatum is a crucial site of action for the motor effects of cannabinoids (CBs). However, the electrophysiological consequences of activation of CB receptors on the striatal neurons have not been established. Here we report for the first time that the cannabimimetic aminoalkylindole WIN 55,212-2 and the endogenous cannabinoid anandamide substantially depress corticostriatal glutamatergic synaptic transmission onto striatal neurons in the brain slice preparation. The selective CB1 receptor antagonist SR 141716 effectively reversed this inhibition. WIN 55,212-2 significantly increased the paired-pulse facilitation of synaptically evoked EPSCs, while having no effect on the sensitivity of postsynaptic neurons to [alpha]-amino-3-hydroxy-5-methylisoxazole-4-propionic acid. WIN 55,212-2 also reduced the frequency of spontaneous, action potential-dependent EPSCs (sEPSCs) without altering their amplitude distribution. Superfusion of WIN 55,212-2 elicited a membrane hyperpolarization accompanied by a decrease in input resistance. Both effects were blocked by intracellular caesium. In contrast, intracellular caesium failed to affect WIN 55,212-2-mediated synaptic inhibition. The WIN 55,212-2-mediated synaptic inhibition was blocked by the Gi/o protein inhibitor pertussis toxin (PTX), but not by the GABA(A) receptor antagonist bicuculline or GABA(B) receptor antagonist SCH 50911. Pretreatment with the N-type Ca2+ channel antagonist [omega]-conotoxin GVIA selectively abolished the WIN-55,212-2-mediated synaptic inhibition. These results suggest that cannabinoids depress the corticostriatal glutamatergic synaptic transmission through the activation of presynaptic CB1 receptors to inhibit N-type Ca2+ channel activity, which in turn reduces glutamate release. The presynaptic action of cannabinoids is mediated by a PTX-sensitive Gi/o protein-coupled signalling pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Action Potentials / drug effects
  • Action Potentials / physiology
  • Analgesics / pharmacology
  • Animals
  • Benzoxazines
  • Cadmium Chloride / pharmacology
  • Calcium Channel Blockers / pharmacology
  • Calcium Channels, N-Type / metabolism
  • Corpus Striatum / cytology*
  • Excitatory Amino Acid Agonists / pharmacology
  • Excitatory Postsynaptic Potentials / drug effects
  • Excitatory Postsynaptic Potentials / physiology
  • GTP-Binding Protein alpha Subunits, Gi-Go / metabolism
  • Glutamic Acid / metabolism
  • Male
  • Morpholines / pharmacology
  • Naphthalenes / pharmacology
  • Neural Inhibition / drug effects
  • Neural Inhibition / physiology
  • Neurons / drug effects
  • Neurons / physiology*
  • Organ Culture Techniques
  • Pertussis Toxin
  • Piperidines / pharmacology
  • Presynaptic Terminals / metabolism*
  • Pyrazoles / pharmacology
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Cannabinoid
  • Receptors, Drug / antagonists & inhibitors
  • Receptors, Drug / metabolism*
  • Receptors, GABA-A / metabolism
  • Receptors, GABA-B / metabolism
  • Rimonabant
  • Synaptic Transmission / drug effects
  • Synaptic Transmission / physiology*
  • Tetrodotoxin / pharmacology
  • Virulence Factors, Bordetella / pharmacology
  • alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / pharmacology

Substances

  • Analgesics
  • Benzoxazines
  • Calcium Channel Blockers
  • Calcium Channels, N-Type
  • Excitatory Amino Acid Agonists
  • Morpholines
  • Naphthalenes
  • Piperidines
  • Pyrazoles
  • Receptors, Cannabinoid
  • Receptors, Drug
  • Receptors, GABA-A
  • Receptors, GABA-B
  • Virulence Factors, Bordetella
  • Glutamic Acid
  • Tetrodotoxin
  • Win 55212-2
  • alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
  • Pertussis Toxin
  • GTP-Binding Protein alpha Subunits, Gi-Go
  • Cadmium Chloride
  • Rimonabant