Genetic transformation of yeast

Biotechniques. 2001 Apr;30(4):816-20, 822-6, 828 passim. doi: 10.2144/01304rv02.


Genetic transformation was first described by Griffith in 1928 and has since been demonstrated in a variety of organisms, including many species of fungi. This review focuses on the history and technology of the transformation of Saccharomyces cerevisiae. The application of protocols developed for S. cerevisiae to other important yeast species is discussed. The protocols for transformation by spheroplasting, LiAc/ssDNA/PEG, and electroporation are compared, and possible mechanisms for transformation are discussed.

Publication types

  • Review

MeSH terms

  • Gene Transfer Techniques*
  • Saccharomyces cerevisiae / genetics*
  • Transformation, Genetic*