High-level Chloroquine Resistance in Sudanese Isolates of Plasmodium Falciparum Is Associated With Mutations in the Chloroquine Resistance Transporter Gene PFCRT and the Multidrug Resistance Gene pfmdr1

J Infect Dis. 2001 May 15;183(10):1535-8. doi: 10.1086/320195. Epub 2001 Apr 13.

Abstract

Polymorphisms were examined in 2 Plasmodium falciparum genes, as were chloroquine responses of clones and isolates from a village in eastern Sudan. There was a significant association between an allele of the P. falciparum chloroquine resistance transporter gene (pfcrt-T76) and both in vitro and in vivo resistance. There was a less significant association with the multidrug resistance gene pfmdr1-Y86 allele. A significant association between pfmdr1-Y86 and pfcrt-T76 was apparent among resistant isolates, which suggests a joint action of the 2 genes in high-level chloroquine resistance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP-Binding Cassette Transporters*
  • Alleles
  • Animals
  • Antimalarials / pharmacology
  • Antimalarials / therapeutic use*
  • Chloroquine / pharmacology
  • Chloroquine / therapeutic use*
  • Clone Cells
  • Drug Resistance
  • Genes, Protozoan
  • Humans
  • Linkage Disequilibrium
  • Malaria, Falciparum / drug therapy*
  • Malaria, Falciparum / parasitology
  • Membrane Proteins / genetics*
  • Membrane Transport Proteins
  • Mutation
  • Parasitic Sensitivity Tests
  • Plasmodium falciparum / drug effects*
  • Plasmodium falciparum / genetics
  • Plasmodium falciparum / isolation & purification
  • Polymorphism, Genetic
  • Protozoan Proteins / genetics*
  • Sudan

Substances

  • ATP-Binding Cassette Transporters
  • Antimalarials
  • Membrane Proteins
  • Membrane Transport Proteins
  • PfCRT protein, Plasmodium falciparum
  • Protozoan Proteins
  • mdr gene protein, Plasmodium
  • Chloroquine