Sparking new frontiers: using in vivo electroporation for genetic manipulations

Dev Biol. 2001 May 1;233(1):13-21. doi: 10.1006/dbio.2001.0181.


In vivo electroporation is a fascinating new approach by which gene expression, regulation, and function can be studied in developmental systems. This technique offers new opportunities for manipulations in animal models that lack genetic approaches, including avians. Furthermore, this approach is applicable to other embryo populations including mice, ascidians, zebrafish, Xenopus, and Drosophila. In this review, we discuss technical aspects of in vivo electroporation, review recent studies where this approach has been utilized successfully, and identify future directions.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Animals
  • Chick Embryo
  • Electroporation / methods*
  • Embryology / methods*
  • Genetic Techniques*
  • In Vitro Techniques
  • Mice
  • Nervous System / embryology