Expression of nm23-H1 gene product in esophageal squamous cell carcinoma and its association with vessel invasion and survival

BMC Cancer. 2001;1:3. doi: 10.1186/1471-2407-1-3. Epub 2001 Apr 19.

Abstract

Background: We assessed the nm23-H1 gene product expression and its relationship with lymphatic and blood vessel invasion in patients with esophageal squamous cell carcinoma.

Methods: Formalin-fixed and paraffin-embedded tissue sections from 45 patients who were treated surgically were used in this study. Pathologists graded lymphatic and blood vessel invasion in each of the tissue samples. Expression of nm23-Hl gene product was determined using a specific monoclonal antibody.

Results: Expression of nm23-H1 gene product was present in 17 (37.8%) cases. We found an inverse correlation between nm23-H1 gene product expression and lymphatic vessel invasion, whereas no correlation between nm23-H1 gene product expression and blood vessel invasion. Overall survival rate was not different between nm23-H1 gene product positive and negative patients (p = 0.21). However, reduced expression of nm23-H1 gene product was associated with shorter overall survival in patients with involved lymph nodes (p < 0.05), but not in patients without involved lymph nodes (p = 0.87).

Conclusions: In patients with esophageal squamous cell carcinoma, there appears to be an inverse relationship between nm23-H1 gene product expression and lymphatic vessel invasion. Furthermore, nm23-H1 gene product expression might be a prognostic marker in patients with involved lymph nodes. Our data does not demonstrate any correlation between nm23-H1 gene product expression and blood vessel invasion.

MeSH terms

  • Biomarkers, Tumor / biosynthesis
  • Biomarkers, Tumor / genetics
  • Carcinoma, Squamous Cell / genetics
  • Carcinoma, Squamous Cell / metabolism*
  • Carcinoma, Squamous Cell / pathology
  • Carcinoma, Squamous Cell / secondary
  • Esophageal Neoplasms / genetics
  • Esophageal Neoplasms / metabolism*
  • Esophageal Neoplasms / pathology*
  • Female
  • Fixatives
  • Formaldehyde
  • Gene Expression Regulation, Neoplastic / genetics
  • Humans
  • Lymphatic Metastasis / genetics
  • Male
  • Middle Aged
  • Monomeric GTP-Binding Proteins / biosynthesis*
  • NM23 Nucleoside Diphosphate Kinases
  • Neoplasm Invasiveness / genetics
  • Neoplasm Invasiveness / pathology
  • Neoplasms, Vascular Tissue / genetics
  • Neoplasms, Vascular Tissue / secondary
  • Nucleoside-Diphosphate Kinase*
  • Paraffin Embedding
  • Random Allocation
  • Survival Analysis
  • Transcription Factors / biosynthesis*

Substances

  • Biomarkers, Tumor
  • Fixatives
  • NM23 Nucleoside Diphosphate Kinases
  • Transcription Factors
  • Formaldehyde
  • NME1 protein, human
  • Nucleoside-Diphosphate Kinase
  • Monomeric GTP-Binding Proteins