zur: a Zn(2+)-responsive regulatory element of Staphylococcus aureus

Microbiology. 2001 May;147(Pt 5):1259-1266. doi: 10.1099/00221287-147-5-1259.


A putative operon encoding a probable zinc-responsive regulatory element (zur) and components of an ABC-type transporter (mreA mreB) have been characterized in Staphylococcus aureus. The zur gene was inactivated but apparently this did not alter Zn(2+) uptake. Expression of mreAB zur is at a low level under a range of ion conditions. To allow inducible expression of the operon, a construct was made placing it under the control of the IPTG-inducible P(spac) promoter. Using this approach, it was shown that zur is able to repress expression of the entire operon in a Zn(2+)-dependent manner, and that mreA and mreB are likely to be involved in high-affinity ion uptake. zur has no apparent role in pathogenicity in a lesion model of S. aureus infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP-Binding Cassette Transporters / genetics*
  • ATP-Binding Cassette Transporters / metabolism
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Biological Transport, Active
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Escherichia coli Proteins*
  • Ion Transport
  • Isopropyl Thiogalactoside
  • Molecular Sequence Data
  • Operon
  • Promoter Regions, Genetic
  • Repressor Proteins / genetics*
  • Repressor Proteins / metabolism
  • Staphylococcal Infections / microbiology
  • Staphylococcus aureus / genetics*
  • Staphylococcus aureus / metabolism
  • Staphylococcus aureus / pathogenicity
  • Zinc / metabolism*


  • ATP-Binding Cassette Transporters
  • Bacterial Proteins
  • DNA-Binding Proteins
  • Escherichia coli Proteins
  • Repressor Proteins
  • Zur protein, E coli
  • MreB protein, E coli
  • Isopropyl Thiogalactoside
  • Zinc

Associated data

  • GENBANK/AF101263