Purification, cloning, and characterization of an arylsulfotransferase from the anaerobic bacterium Eubacterium rectale IIIH

S L Goldberg; V Nanduri; P M Cino; R Patel

doi:10.1038/sj.jim.7000084

Purification, cloning, and characterization of an arylsulfotransferase from the anaerobic bacterium Eubacterium rectale IIIH

J Ind Microbiol Biotechnol. 2000 Dec;25(6):305-309. doi: 10.1038/sj.jim.7000084.

Authors

S L Goldberg¹, V Nanduri, P M Cino, R Patel

Affiliation

¹ Pharmaceutical Research Institute, Pharmaceutical Research and Development, Bristol-Myers Squibb Co., New Brunswick, NJ 08903.

PMID: 11320417
DOI: 10.1038/sj.jim.7000084

Abstract

A bacterium, Eubacterium rectale IIIH, which possessed arylsulfotransferase (ASST) activity was isolated from human feces. The ASST gene (astA) was cloned and the corresponding protein partially characterized. This gene shows only moderate homology to the previously sequenced ASST genes of Klebsiella and Enterobacter, which are very closely related to each other.