Effects of the green algae Chlorella vulgaris on the response of the host hematopoietic system to intraperitoneal ehrlich ascites tumor transplantation in mice

Immunopharmacol Immunotoxicol. 2001 Feb;23(1):119-32. doi: 10.1081/iph-100102573.


Chlorella vulgaris extract (CVE) was examined for its effects on the Ehrlich ascites tumor-induced suppression in the numbers of bone marrow and spleen granulocyte-macrophage progenitor cells (CFU-GM) in mice. No effects on bone marrow and spleen CFU-GM, as compared to controls, were observed in normal mice given 50, 100 and 200 mg/kg CVE orally for 5 days. In tumor-bearing mice, myelosuppression concomitant with increased number of spleen CFU-GM were observed. The number of CFU-GM in the bone marrow was restored to control levels after the administration of CVE (50, 100 and 200 mg/kg) to tumor-bearing mice, and a slight reduction in spleen colony formation was observed in these animals. In addition, CVE significantly prolonged the survival of mice inoculated with the Ehrlich ascites tumor. These results suggest a protective antitumor effect of CVE which might be attributable, at least in part, to the stimulation of the production and, possibly, maturation of granulocytes and macrophages.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Oral
  • Animals
  • Bone Marrow Cells / drug effects*
  • Carcinoma, Ehrlich Tumor / immunology*
  • Cells, Cultured
  • Chlorella*
  • Colony-Forming Units Assay
  • Germ-Free Life
  • Granulocyte-Macrophage Colony-Stimulating Factor / drug effects*
  • Hematopoiesis, Extramedullary / drug effects
  • Hematopoietic Stem Cells / drug effects*
  • Injections, Intraperitoneal
  • Leukopoiesis / drug effects*
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Neoplasm Transplantation
  • Plant Extracts / analysis
  • Plant Extracts / pharmacology*
  • Recombinant Proteins
  • Spleen / cytology
  • Survival Rate


  • Plant Extracts
  • Recombinant Proteins
  • Granulocyte-Macrophage Colony-Stimulating Factor