Human septin 3 on chromosome 22q13.2 is upregulated by neuronal differentiation

Biochem Biophys Res Commun. 2001 Apr 27;283(1):48-56. doi: 10.1006/bbrc.2001.4741.


An expression sequence tag identified in a screen for genes upregulated by retinoic acid induced neuronal differentiation of the human teratocarcinoma cell line Ntera2/D1 was found in close genomic proximity to a region of high sequence homology to the septin subfamily of GTPase genes. We could show that the tag corresponds to the 3' untranslated region of this novel gene named septin 3 and cloned three isoforms A (2191 bp), B (4378 bp), and C (1896 bp) from human Ntera2/D1 cDNA. We present the genomic localization and organization on chromosome 22q13.2, a chromosomal hot spot for translocations implicated in leukemia. Interestingly, MSF the closest paralog of septin 3 is a fusion partner in a therapy-related acute myeloid leukemia. Quantitative PCR confirmed the upregulation of the putative septin by neuronal differentiation and northern blotting showed only one band corresponding to sep3B with a neurospecific expression pattern in adult human tissues.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Differentiation / drug effects
  • Cell Differentiation / genetics
  • Chromosomes, Human, Pair 22 / genetics*
  • Cloning, Molecular
  • GTP Phosphohydrolases / genetics
  • GTP Phosphohydrolases / metabolism*
  • Humans
  • Molecular Sequence Data
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism
  • Neurons / cytology
  • Neurons / metabolism*
  • Organ Specificity
  • Phylogeny
  • Physical Chromosome Mapping
  • Protein Isoforms / genetics
  • Protein Isoforms / metabolism
  • Septins
  • Sequence Homology, Amino Acid
  • Teratocarcinoma / genetics
  • Teratocarcinoma / metabolism
  • Tretinoin / pharmacology
  • Tumor Cells, Cultured


  • Nerve Tissue Proteins
  • Protein Isoforms
  • Tretinoin
  • GTP Phosphohydrolases
  • Septins
  • septin 3

Associated data

  • GENBANK/AF285107
  • GENBANK/AF285108
  • GENBANK/AF285109