Methylation of the hMLH1, p16, and MDR1 genes in colorectal carcinoma: associations with clinicopathological features

Cancer Lett. 2001 Jun 10;167(1):91-7. doi: 10.1016/s0304-3835(01)00431-1.

Abstract

The methylation status of seven cancer-related genes was investigated in a series of 58 colorectal cancers, 18 of which showed the microsatellite instability (MSI+) phenotype. Methylation of the hMLH1, p16 and MDR1 genes was found in 23, 29 and 28% of tumors, respectively. None of the tumors showed methylation of the TS, ATM, PARP or p21 genes. Methylation of the hMLH1, p16 and MDR1 genes was more frequent and more concordant in MSI+ compared to MSI- tumors (P<0.001) and was also strongly associated with poor histological differentiation (P<0.001). There were trends for associations between methylation at one or more of these loci and proximal tumor location, advanced Dukes' stage and the presence of wild-type p53 (P=0.06 for each).

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Adult
  • Aged
  • Ataxia Telangiectasia Mutated Proteins
  • Carrier Proteins
  • Cell Cycle Proteins
  • Colorectal Neoplasms / genetics*
  • Colorectal Neoplasms / metabolism
  • Colorectal Neoplasms / pathology
  • CpG Islands / genetics
  • Cyclin-Dependent Kinase Inhibitor p21
  • Cyclins / genetics
  • DNA Methylation*
  • DNA Primers
  • DNA-Binding Proteins
  • Female
  • Genes, MDR*
  • Genes, p16*
  • Humans
  • Male
  • Microsatellite Repeats / genetics
  • Middle Aged
  • MutL Protein Homolog 1
  • Neoplasm Proteins / genetics*
  • Nuclear Proteins
  • Poly(ADP-ribose) Polymerases / genetics
  • Polymorphism, Single-Stranded Conformational
  • Protein-Serine-Threonine Kinases / genetics
  • Thymidylate Synthase / genetics
  • Tumor Suppressor Proteins

Substances

  • Adaptor Proteins, Signal Transducing
  • CDKN1A protein, human
  • Carrier Proteins
  • Cell Cycle Proteins
  • Cyclin-Dependent Kinase Inhibitor p21
  • Cyclins
  • DNA Primers
  • DNA-Binding Proteins
  • MLH1 protein, human
  • Neoplasm Proteins
  • Nuclear Proteins
  • Tumor Suppressor Proteins
  • Thymidylate Synthase
  • Poly(ADP-ribose) Polymerases
  • ATM protein, human
  • Ataxia Telangiectasia Mutated Proteins
  • Protein-Serine-Threonine Kinases
  • MutL Protein Homolog 1