Dimethylamiloride-induced differentiation of HL-60 cells

Y Y Li; N C Liang; L Jiang; T Wu; X Y Lin

Dimethylamiloride-induced differentiation of HL-60 cells

Acta Pharmacol Sin. 2000 May;21(5):445-9.

Authors

Y Y Li¹, N C Liang, L Jiang, T Wu, X Y Lin

Affiliation

¹ Institute of Medical Biochemistry, Guangdong Medical College, Zhanjiang, Guangdong 524023, China. Guangdong Key Lab for Research & Development of Natural Medicines, Zhanjiang, Guangdong 524023, China. liyunyuan@163.net

PMID: 11324444

Abstract

Aim: To study the effect of 5-(N,N-dimethyl) amiloride (DMA) on the proliferation and differentiation of HL-60 cells in vitro.

Methods: MTT assay to test cytotoxicity; cell staining and NBT reduction to test cell differentiation.

Results: DMA inhibited HL-60 cells growth in a concentration-dependent manner, and IC50 value for 96 h was 31.7 (95% confidence limits: 6.3-57.1) mumol.L-1. DMA also induced granulocytic differentiation in HL-60 cells. The percentage of differentiating cells increased from 6.5% to 70% after DMA 100 mumol.L-1 treatment for 3 d. The differentiating effect of DMA was distinguishable from amiloride, 5-(N-ethyl-N-isopropyl)amiloride (EIPA), and (5-(N-ethyl-N-isopropyl)amiloride (MIA). None among the amiloride, EIPA, and MIA were capable of triggering the differentiation of HL-60 cells.

Conclusion: DMA inhibited the proliferation of HL-60 cells and induced differentiation of HL-60 cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amiloride / analogs & derivatives
Amiloride / pharmacology*
Cell Division / drug effects
Cell Transformation, Neoplastic / drug effects*
HL-60 Cells / drug effects
Humans
Inhibitory Concentration 50

Substances

5-dimethylamiloride
Amiloride