Does Blood of Healthy Subjects Contain Bacterial Ribosomal DNA?

J Clin Microbiol. 2001 May;39(5):1956-9. doi: 10.1128/JCM.39.5.1956-1959.2001.

Abstract

Real-time PCR methods with primers and a probe targeting conserved regions of the bacterial 16S ribosomal DNA (rDNA) revealed a larger amount of rDNA in blood specimens from healthy individuals than in matched reagent controls. However, the origins and identities of these blood-associated bacterial rDNA sequences remain obscure.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacteria / genetics
  • DNA Primers / genetics
  • DNA, Bacterial / blood*
  • DNA, Ribosomal / blood*
  • Humans
  • Polymerase Chain Reaction / methods*
  • RNA, Ribosomal, 16S / genetics*

Substances

  • DNA Primers
  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S