Real-time PCR assay targeting IS481 of Bordetella pertussis and molecular basis for detecting Bordetella holmesii

J Clin Microbiol. 2001 May;39(5):1963-6. doi: 10.1128/JCM.39.5.1963-1966.2001.


Detection of Bordetella holmesii by a real-time PCR assay targeting IS481 of Bordetella pertussis is reported. Sequencing of IS481-specific PCR products from B. pertussis and B. holmesii isolates revealed sequence homology. Restriction fragment length polymorphism demonstrated a low copy number of IS481-like sequences in B. holmesii. These results, and culture of B. holmesii from patients with cough, suggest that the specificity and predictive value of IS481-based PCR assays for pertussis may be compromised.

Publication types

  • Evaluation Study

MeSH terms

  • Bordetella / classification*
  • Bordetella / genetics
  • Bordetella / isolation & purification
  • Bordetella Infections / diagnosis
  • Bordetella Infections / microbiology*
  • Bordetella pertussis / genetics*
  • DNA Transposable Elements / genetics*
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods*
  • Polymorphism, Restriction Fragment Length
  • Sensitivity and Specificity


  • DNA Transposable Elements

Associated data

  • GENBANK/AF349431