15-Deoxy-delta(12,14)-prostaglandin J(2) inhibits IL-10 and IL-12 production by macrophages

Biochem Biophys Res Commun. 2001 May 4;283(2):344-6. doi: 10.1006/bbrc.2001.4783.


15-Deoxy-Delta(12,14)-prostaglandin J(2) (dPGJ(2)) is a metabolite of prostaglandin D(2), that binds to peroxisome proliferator-activated receptor gamma (PPARgamma). PPARgamma and prostaglandin D(2) synthase, which is required for dPGJ(2) synthesis, are predominantly expressed in macrophages. In contrast, IL-10 and IL-12 produced by macrophages stimulate Th1 and Th2 immune response, respectively. This study investigated the effect of dPGJ(2) on IL-10 and IL-12 production by macrophages in response to lipopolysaccharide (LPS). Our data clearly demonstrated that dPGJ(2) inhibits LPS-induced IL-10 and IL-12 production by macrophages. A different agonist of PPARgamma, 13-hydroxyoctadecadienoic acid, similarly inhibited the production of IL-10 and IL-12 in response to LPS. Further, dPGJ(2) did not appear to act through the PGD(2) receptor. These results suggest that dPGJ(2) may inhibit LPS-induced IL-10 and IL-12 production by macrophages through PPARgamma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • In Vitro Techniques
  • Interleukin-10 / biosynthesis*
  • Interleukin-12 / biosynthesis*
  • Linoleic Acids / pharmacology
  • Lipopolysaccharides / pharmacology
  • Macrophages, Peritoneal / drug effects*
  • Macrophages, Peritoneal / immunology*
  • Macrophages, Peritoneal / metabolism
  • Male
  • Prostaglandin D2 / analogs & derivatives
  • Prostaglandin D2 / pharmacology*
  • Rats
  • Rats, Wistar
  • Receptors, Cytoplasmic and Nuclear / agonists
  • Receptors, Cytoplasmic and Nuclear / metabolism
  • Receptors, Immunologic*
  • Receptors, Prostaglandin / drug effects
  • Receptors, Prostaglandin / metabolism
  • Transcription Factors / agonists
  • Transcription Factors / metabolism


  • 15-deoxy-delta(12,14)-prostaglandin J2
  • Linoleic Acids
  • Lipopolysaccharides
  • Receptors, Cytoplasmic and Nuclear
  • Receptors, Immunologic
  • Receptors, Prostaglandin
  • Transcription Factors
  • Interleukin-10
  • Interleukin-12
  • 13-hydroxy-9,11-octadecadienoic acid
  • Prostaglandin D2
  • prostaglandin D2 receptor