Four cell lines originated from embryo, caudal fin, anal fin and snout of Colossoma brachypomum were established, and subcultured for 70, 47, 45 and 40 passages to the present. The results of the experiment indicated that the four cell lines were fibroblast-like diploid cells, and the model number of their chromosome is 54. These cell lines could grow under temperature of 20-35 degrees C, and its optimum temperature was 27-30 degrees C. The cells grew slowly at 20 degrees C, stopped growth at 15 degrees C, and died below 10 degrees C. This indicated that the growth temperature of the these cell lines was coincident with that of fish. In addition, the attachment time, doubling time, mitotic index, passage survival rate and cell cycle were determined and compared.