Analysis of epidermal-type transglutaminase (TGase 3) expression in mouse tissues and cell lines

Int J Biochem Cell Biol. 2001 May;33(5):491-8. doi: 10.1016/s1357-2725(01)00033-4.


In the formation of the cornified cell envelope in the epidermis, epidermal-type transglutaminase (TGase 3) cross-links a variety of structural proteins. However, its expression in other tissue has not been investigated. Furthermore, no cell line expressing TGase 3 has been found. The tissue distribution of TGase 3 in mice was investigated using reverse-transcription polymerase chain reaction (RT-PCR) and Western blotting analyses. TGase 3 mRNA was expressed in the brain, stomach, spleen, small intestine, testis, skeletal muscle and skin. The stomach and testis expressed TGase 3 protein in size similar to that observed in the epidermis. Screening various cell lines, a gastric human cancer cell line, MKN-1 and mouse neuroblast cell line, neuro2a, were found to express TGase 3.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Calcium-Binding Proteins / genetics
  • Calcium-Binding Proteins / metabolism*
  • Cell Line
  • Cells, Cultured
  • Epidermal Cells
  • Epidermis / enzymology*
  • Epidermis / physiology
  • Humans
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transglutaminases / genetics
  • Transglutaminases / metabolism*


  • Calcium-Binding Proteins
  • Tgm3 protein, mouse
  • TGM3 protein, human
  • Transglutaminases