Measurement of nicotine in hair by reversed-phase high-performance liquid chromatography with electrochemical detection

J Chromatogr B Biomed Sci Appl. 2001 Apr 5;753(2):179-87. doi: 10.1016/s0378-4347(00)00540-5.


We have developed an assay for nicotine in hair based on reversed-phase HPLC with electrochemical detection. The method uses a low-metal, high-purity silica reversed-phase column. We have investigated the washing, digestion and extraction procedures and discuss the important points in the HPLC method development. The assay is presented as an application in a population of exposed and non-exposed children. Analytical parameters are satisfactory with linearity, recoveries, limit of quantitation and precision all suitable for epidemiological studies involving environmental tobacco smoke exposure assessment.

MeSH terms

  • Child, Preschool
  • Chromatography, High Pressure Liquid / methods*
  • Electrochemistry / methods*
  • Environmental Exposure
  • Hair / chemistry*
  • Humans
  • Infant
  • Nicotine / analysis*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Tobacco Smoke Pollution


  • Tobacco Smoke Pollution
  • Nicotine