Quantitative determination of urinary 8-hydroxydeoxyguanosine (8-OH-dg) by using ELISA

Res Commun Mol Pathol Pharmacol. 2000;107(1-2):39-44.


In order to establish a quantitative assay for 8-OHdG concentrations in urine, we examined the precision of a test for the recovery of 8-OHdG in urine by using an ELISA method. The coefficient of variation (CV) for assay with incubation in water or air ranged between 7.0% and 8.4% and between 19.2% and 30.6%, respectively. The data by using incubation in water gave higher accuracy than those in air. The recovery rates of 8-OHdG in urine sample ranged from 95 - 114%. Our results indicated that the excellent sensitivity of this ELISA kit by using incubation in water makes its use possible for the determination in urine with a good reproducibility and recovery of 8-OHdG-spiked samples.

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Adult
  • Biomarkers / urine*
  • Deoxyguanosine / analogs & derivatives*
  • Deoxyguanosine / urine*
  • Enzyme-Linked Immunosorbent Assay*
  • Humans
  • Male
  • Middle Aged
  • Sensitivity and Specificity


  • Biomarkers
  • 8-Hydroxy-2'-Deoxyguanosine
  • Deoxyguanosine