Drosophila Wingless and Pair-Rule Transcripts Localize Apically by Dynein-Mediated Transport of RNA Particles

Cell. 2001 Apr 20;105(2):209-19. doi: 10.1016/s0092-8674(01)00312-9.

Abstract

Asymmetric mRNA localization targets proteins to their cytoplasmic site of function. We have elucidated the mechanism of apical localization of wingless and pair-rule transcripts in the Drosophila blastoderm embryo by directly visualizing intermediates along the entire path of transcript movement. After release from their site of transcription, mRNAs diffuse within the nucleus and are exported to all parts of the cytoplasm, regardless of their cytoplasmic destinations. Endogenous and injected apical RNAs assemble selectively into cytoplasmic particles that are transported apically along microtubules. Cytoplasmic dynein is required for correct localization of endogenous transcripts and apical movement of injected RNA particles. We propose that dynein-dependent movement of RNA particles is a widely deployed mechanism for mRNA localization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Active Transport, Cell Nucleus / drug effects
  • Active Transport, Cell Nucleus / physiology*
  • Animals
  • Antineoplastic Agents, Phytogenic / pharmacology
  • Cell Polarity*
  • DNA-Binding Proteins / metabolism
  • Demecolcine / pharmacology
  • Drosophila Proteins*
  • Drosophila melanogaster / physiology
  • Dyneins / genetics
  • Dyneins / metabolism*
  • Embryo, Nonmammalian / cytology
  • Embryo, Nonmammalian / physiology*
  • Fluorescent Dyes / metabolism
  • Fushi Tarazu Transcription Factors
  • Homeodomain Proteins / metabolism
  • In Situ Hybridization, Fluorescence
  • Microinjections
  • Microscopy, Fluorescence
  • Microtubules / metabolism*
  • Nuclear Proteins
  • Proto-Oncogene Proteins / metabolism*
  • RNA, Messenger / metabolism*
  • Time Factors
  • Transcription Factors
  • Wnt1 Protein

Substances

  • Antineoplastic Agents, Phytogenic
  • DNA-Binding Proteins
  • Drosophila Proteins
  • Fluorescent Dyes
  • Fushi Tarazu Transcription Factors
  • Homeodomain Proteins
  • Nuclear Proteins
  • Proto-Oncogene Proteins
  • RNA, Messenger
  • Transcription Factors
  • Wnt1 Protein
  • ftz protein, Drosophila
  • run protein, Drosophila
  • wg protein, Drosophila
  • Dyneins
  • Demecolcine