A Rsc3/Rsc30 zinc cluster dimer reveals novel roles for the chromatin remodeler RSC in gene expression and cell cycle control

Mol Cell. 2001 Apr;7(4):741-51. doi: 10.1016/s1097-2765(01)00219-2.


Chromatin remodeling complexes perform central roles in transcriptional regulation. Here, we identify Rsc3 and Rsc30 as novel components of the essential yeast remodeler RSC complex. Rsc3 and Rsc30 function requires their zinc cluster domain, a known site-specific DNA binding motif. RSC3 is essential, and rsc3 Ts- mutants display a G2/M cell cycle arrest involving the spindle assembly checkpoint pathway, whereas rsc30Delta mutants are viable and osmosensitive. Rsc3 and Rsc30 interact functionally and also physically as a stable Rsc3/Rsc30 heteromeric complex. However, DNA microarray analysis with rsc3 or rsc30 mutants reveals different effects on the expression levels of ribosomal protein genes and cell wall genes. We propose that Rsc3 and Rsc30 interact physically but have different roles in targeting or regulating RSC.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Cell Cycle / genetics
  • Cell Wall / physiology
  • Chromatin / genetics
  • Chromatin / metabolism*
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Dimerization
  • G2 Phase / genetics
  • Gene Expression Regulation, Fungal*
  • Genes, Lethal / physiology
  • Genes, cdc / physiology
  • Leucine Zippers / physiology
  • Mitosis / genetics
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Nuclear Proteins / genetics
  • Protein Structure, Secondary
  • Protein Structure, Tertiary
  • Ribosomal Proteins / genetics
  • Ribosomal Proteins / metabolism
  • Saccharomyces cerevisiae Proteins*
  • Spindle Apparatus / genetics
  • Spindle Apparatus / metabolism
  • Temperature
  • Transcription Factors / chemistry
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism
  • Yeasts
  • Zinc / chemistry
  • Zinc / metabolism*


  • Chromatin
  • DNA-Binding Proteins
  • Nuclear Proteins
  • PAF1 protein, S cerevisiae
  • RSC complex, S cerevisiae
  • Ribosomal Proteins
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Zinc