Stem cell factor and igE-stimulated murine mast cells produce chemokines (CCL2, CCL17, CCL22) and express chemokine receptors

Inflamm Res. 2001 Mar;50(3):168-74. doi: 10.1007/s000110050741.


Objective and design: In the present study we investigated the effect of SCF and/or IgE on histamine, TNF-alpha and chemokines released from bone marrow-derived mast cells (BMMC) as well as chemokine receptor expression.

Material and methods: BMMC were derived from femoral bone marrow of CBA/J mice. The purity of BMMC was >98% after 3 weeks. BMMC (2.5 x 10(6) cells/well) were incubated in the presence or absence of either SCF, IgE plus DNP or a combination of SCF and IgE for 6 and 18 h. Cell-free supernatants were recovered to measure CC chemokines, TNF-alpha and histamine release utilizing ELISA assays. CC chemokine family receptors were detected by RT-PCR analysis, and confirmed using functional chemotactic assays.

Results: Histamine levels were comparable between SCF and IgE stimulated cells, whereas TNF-alpha production was significantly greater after IgE compared to SCF stimulation. SCF and/or IgE-stimulated BMMC released CC chemokines, CCL22 (MDC), CCL17 (TARC) and CCL2 (MCP-1). Increased mRNA expression of CCR1, CCR2, CCR3, and CCR5 was detected in SCF and IgE-stimulated BMMCs. Functional chemotactic assays confirmed the expression data.

Conclusion: SCF and IgE can up-regulate the expression of chemokines and chemokine receptors on mast cells. Thus, SCF may play a significant role in their activation and inflammation during allergic responses.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Chemokines, CC / biosynthesis*
  • Chemotaxis
  • Histamine Release / drug effects
  • Immunoglobulin E / pharmacology*
  • Mast Cells / metabolism*
  • Mice
  • Mice, Inbred CBA
  • Receptors, Chemokine / biosynthesis*
  • Stem Cell Factor / pharmacology*
  • Tumor Necrosis Factor-alpha / biosynthesis


  • Chemokines, CC
  • Receptors, Chemokine
  • Stem Cell Factor
  • Tumor Necrosis Factor-alpha
  • Immunoglobulin E