Compact myelin dictates the differential targeting of two sodium channel isoforms in the same axon

Neuron. 2001 Apr;30(1):91-104. doi: 10.1016/s0896-6273(01)00265-3.


Voltage-dependent sodium channels are uniformly distributed along unmyelinated axons, but are highly concentrated at nodes of Ranvier in myelinated axons. Here, we show that this pattern is associated with differential localization of distinct sodium channel alpha subunits to the unmyelinated and myelinated zones of the same retinal ganglion cell axons. In adult axons, Na(v)1.2 is localized to the unmyelinated zone, whereas Na(v)1.6 is specifically targeted to nodes. During development, Na(v)1.2 is expressed first and becomes clustered at immature nodes of Ranvier, but as myelination proceeds, Na(v)1.6 replaces Na(v)1.2 at nodes. In Shiverer mice, which lack compact myelin, Na(v)1.2 is found throughout adult axons, whereas little Na(v)1.6 is detected. Together, these data show that sodium channel isoforms are differentially targeted to distinct domains of the same axon in a process associated with formation of compact myelin.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Axons / metabolism*
  • Axons / ultrastructure
  • Immunohistochemistry
  • Mice
  • Mice, Neurologic Mutants / anatomy & histology
  • Mice, Neurologic Mutants / growth & development
  • Mice, Neurologic Mutants / metabolism
  • Myelin Sheath / metabolism*
  • Myelin Sheath / ultrastructure
  • Optic Nerve / growth & development*
  • Optic Nerve / metabolism
  • Optic Nerve / ultrastructure
  • Peripheral Nerves / growth & development
  • Peripheral Nerves / metabolism
  • Peripheral Nerves / ultrastructure
  • Protein Isoforms / metabolism
  • Protein Isoforms / ultrastructure
  • Ranvier's Nodes / metabolism
  • Ranvier's Nodes / ultrastructure
  • Rats
  • Rats, Sprague-Dawley
  • Retinal Ganglion Cells / metabolism
  • Retinal Ganglion Cells / ultrastructure
  • Sodium Channels / genetics
  • Sodium Channels / metabolism*


  • Protein Isoforms
  • Sodium Channels