A 28 kDa protein of the Bacillus thuringiensis serovar shandongiensis isolate 89-T-34-22 induces a human leukemic cell-specific cytotoxicity

Biochim Biophys Acta. 2001 May 5;1547(1):57-63. doi: 10.1016/s0167-4838(01)00169-8.

Abstract

A 28 kDa protein that exhibits cytocidal activity specific for human leukemic T (MOLT-4) cells was purified from proteinase K-digested parasporal inclusion of a Bacillus thuringiensis serovar shandongiensis isolate. The N-terminal sequence of the protein was identical with that of the 32 kDa protein, regarded as a protoxin, of the inclusion proteins. The median effective concentration of this protein was 0.23 microg/ml against MOLT-4 cells and its specific activity was 7.9 times greater than that of the whole inclusion proteins. The 28 kDa protein induced necrosis-like cytotoxicity against MOLT-4 cells and the cytopathic effect with the passage of time was characterized by cell swelling, nuclear membrane isolation and chromatin condensation.

Publication types

  • Comparative Study

MeSH terms

  • Antineoplastic Agents / pharmacology
  • Bacillus thuringiensis / chemistry
  • Bacillus thuringiensis / isolation & purification*
  • Bacterial Proteins / isolation & purification*
  • Bacterial Proteins / pharmacology
  • Cell Nucleus / drug effects
  • Chemical Fractionation
  • Chromatography, Ion Exchange
  • Crotalid Venoms
  • Electrophoresis, Polyacrylamide Gel
  • Endopeptidase K
  • Endotoxins / isolation & purification
  • HeLa Cells / drug effects
  • Humans
  • Leukemia, T-Cell
  • Mitochondrial Swelling / drug effects
  • Naphthols
  • Neurotoxins
  • Nuclear Envelope / drug effects
  • Tumor Cells, Cultured / drug effects

Substances

  • Antineoplastic Agents
  • Bacterial Proteins
  • Crotalid Venoms
  • Endotoxins
  • Mojave toxin
  • Naphthols
  • Neurotoxins
  • parasporin
  • AA-497
  • Endopeptidase K