Spliceosomal UsnRNP biogenesis, structure and function

Curr Opin Cell Biol. 2001 Jun;13(3):290-301. doi: 10.1016/s0955-0674(00)00211-8.

Abstract

Significant advances have been made in elucidating the biogenesis pathway and three-dimensional structure of the UsnRNPs, the building blocks of the spliceosome. U2 and U4/U6*U5 tri-snRNPs functionally associate with the pre-mRNA at an earlier stage of spliceosome assembly than previously thought, and additional evidence supporting UsnRNA-mediated catalysis of pre-mRNA splicing has been presented.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Crystallography
  • Humans
  • Macromolecular Substances
  • Protein Structure, Tertiary / physiology
  • Protein Transport / physiology
  • RNA Splicing / genetics
  • RNA Splicing / physiology
  • RNA, Small Nuclear / chemistry
  • RNA, Small Nuclear / genetics
  • RNA, Small Nuclear / metabolism*
  • Ribonucleoprotein, U1 Small Nuclear / biosynthesis*
  • Ribonucleoprotein, U1 Small Nuclear / chemistry
  • Ribonucleoprotein, U1 Small Nuclear / genetics
  • Ribonucleoproteins, Small Nuclear / biosynthesis*
  • Ribonucleoproteins, Small Nuclear / chemistry
  • Ribonucleoproteins, Small Nuclear / genetics
  • Spliceosomes / chemistry
  • Spliceosomes / genetics*
  • Spliceosomes / metabolism*

Substances

  • Macromolecular Substances
  • RNA, Small Nuclear
  • Ribonucleoprotein, U1 Small Nuclear
  • Ribonucleoproteins, Small Nuclear
  • U4 small nuclear RNA