Enhanced systemic and mucosal antibody responses to a model protein antigen after intranasal and intratracheal immunisation using Bacillus firmus as an adjuvant

Immunol Lett. 2001 May 1;77(1):39-45. doi: 10.1016/s0165-2478(01)00192-4.

Abstract

Bacillus firmus, a non-pathogenic Gram positive (G+) bacterium of the external environment was investigated for immunomodulatory properties. It stimulated an increase in anti-ovalbumin IgG in sera, bronchoalveolar lavages and intestinal washings after both intranasal (i.n.) and intratracheal (i.t.) immunisation, and enhanced anti-ovalbumin IgA in intestinal secretions and in bronchoalveolar lavage fluid after i.n. or i.t. immunisation, respectively. The immunomodulatory effect of B. firmus on antibody formation was antigen specific.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic*
  • Administration, Intranasal
  • Animals
  • Antibodies / immunology*
  • Antibodies, Bacterial / blood
  • Antibody Formation
  • Antigens / immunology
  • Bacillus / immunology*
  • Bronchoalveolar Lavage Fluid / immunology
  • Female
  • Immunity, Mucosal* / immunology
  • Injections
  • Intestinal Mucosa / immunology*
  • Intestines / immunology
  • Mice
  • Mice, Inbred BALB C
  • Ovalbumin / immunology
  • Respiratory Mucosa / immunology*
  • Trachea

Substances

  • Adjuvants, Immunologic
  • Antibodies
  • Antibodies, Bacterial
  • Antigens
  • Ovalbumin