Identified neurons in C. elegans coexpress vesicular transporters for acetylcholine and monoamines

Am J Physiol Cell Physiol. 2001 Jun;280(6):C1616-22. doi: 10.1152/ajpcell.2001.280.6.C1616.

Abstract

We have identified four neurons (VC4, VC5, HSNL, HSNR) in Caenorhabditis elegans adult hermaphrodites that express both the vesicular acetylcholine transporter and the vesicular monoamine transporter. All four of these cells are motor neurons that innervate the egg-laying muscles of the vulva. In addition, they all express choline acetyltransferase, the synthetic enzyme for acetylcholine. The distributions of the vesicular acetylcholine transporter and the vesicular monoamine transporter are not identical within the individual cells. In mutants deficient for either of these transporters, there is no apparent compensatory change in the expression of the remaining transporter. This is the first report of neurons that express two different vesicular neurotransmitter transporters in vivo.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Caenorhabditis elegans
  • Carrier Proteins / analysis
  • Carrier Proteins / biosynthesis
  • Carrier Proteins / metabolism*
  • Fluorescent Antibody Technique
  • Membrane Glycoproteins / analysis
  • Membrane Glycoproteins / biosynthesis
  • Membrane Glycoproteins / metabolism*
  • Membrane Transport Proteins*
  • Neurons / chemistry
  • Neurons / metabolism*
  • Neuropeptides*
  • Serotonin / analysis
  • Serotonin / metabolism
  • Synaptic Vesicles / chemistry
  • Synaptic Vesicles / metabolism*
  • Vesicular Acetylcholine Transport Proteins
  • Vesicular Biogenic Amine Transport Proteins
  • Vesicular Monoamine Transport Proteins
  • Vesicular Transport Proteins*

Substances

  • Carrier Proteins
  • Membrane Glycoproteins
  • Membrane Transport Proteins
  • Neuropeptides
  • Vesicular Acetylcholine Transport Proteins
  • Vesicular Biogenic Amine Transport Proteins
  • Vesicular Monoamine Transport Proteins
  • Vesicular Transport Proteins
  • Serotonin