Immunohistochemical Analysis of NY-ESO-1 Antigen Expression in Normal and Malignant Human Tissues

Int J Cancer. 2001 Jun 15;92(6):856-60. doi: 10.1002/ijc.1282.

Abstract

NY-ESO-1, a member of the CT (cancer/testis) family of antigens, is expressed in normal testis and in a range of human tumor types. Knowledge of NY-ESO-1 expression has depended on RT-PCR detection of mRNA and there is a need for detecting NY-ESO-1 at the protein level. In the present study, a method for the immunochemical detection of NY-ESO-1 in paraffin-embedded tissues has been developed and used to define the expression pattern of NY-ESO-1 in normal tissues and in a panel of human tumors. No normal tissue other than testis showed NY-ESO-1 reactivity, and expression in testis was restricted to germ cells particularly spermatogonia. In human tumors, the frequency of NY-ESO-1 antigen expression corresponds with past analysis of NY-ESO-1 mRNA expression e.g., 20-30% of lung cancers, bladder cancers and melanoma, and no expression in colon and renal cancer. Co-typing of NY-ESO-1 antigen and mRNA expression in a large panel of lung cancers showed a good correlation. There is great variability in NY-ESO-1 expression in individual tumors, ranging from an infrequent homogeneous pattern of staining to highly heterogeneous antigen expression.

MeSH terms

  • Antibodies, Monoclonal / metabolism
  • Antigens, Neoplasm*
  • Breast Neoplasms / metabolism
  • Carcinoma, Renal Cell / metabolism
  • Colonic Neoplasms / metabolism
  • Female
  • Head and Neck Neoplasms / metabolism
  • Humans
  • Immunohistochemistry
  • Kidney Neoplasms / metabolism
  • Lung Neoplasms / metabolism
  • Male
  • Melanoma / metabolism
  • Membrane Proteins*
  • Protein Biosynthesis*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Spermatogonia / metabolism
  • Testis / metabolism*
  • Tissue Distribution
  • Tumor Cells, Cultured
  • Urinary Bladder Neoplasms / metabolism

Substances

  • Antibodies, Monoclonal
  • Antigens, Neoplasm
  • CTAG1B protein, human
  • Membrane Proteins